Review



pet30a expression vector  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Millipore pet30a expression vector
    Pet30a Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30a+expression+vector/pet28a/pmc03464273-227-0-3
    Average 90 stars, based on 1 article reviews
    pet30a expression vector - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Sequencing:

    Article Title: Nuclear DNA Sensor IFI16 as Circulating Protein in Autoimmune Diseases Is a Signal of Damage that Impairs Endothelial Cells through High-Affinity Membrane Binding
    Article Snippet: Low passage human dermal fibroblasts, HDF (ATCC), mouse fibroblasts, 3T3 (ATCC), HeLa (ATCC) and HaCaT (ATCC) cells were grown in Dulbecco’s modified Eagle’s medium ( DMEM) (Sigma-Aldrich, Milan, Italy) supplemented with 10% fetal bovine serum and 2% Penicillin-Streptomycin solution Unless specified, all cells were grown at 37°C and 5% CO 2 . .. The entire coding sequence of the b isoform of human IFI16 was subcloned into the pET30a expression vector (Novagen, Madison, WI) containing an N-terminal histidine tag. ..

    Article Title: Effects of Hepatitis C core protein on mitochondrial electron transport and production of reactive oxygen species
    Article Snippet: .. Sequence of the cloned PCR fragment was confirmed by DNA sequencing and the fragment was then subcloned into a pET30a expression vector (Novagen, Gillstown, NJ). ..

    Article Title: Method for detecting cancer using CAPRIN-1 as a marker
    Article Snippet: .. Then the gene sequence of interest was inserted into a pET30a expression vector (Novagen) for Escherichia coli treated with Sac I and Xho I restriction enzymes. .. A His tag-fused recombinant protein can be produced using the vector.

    Expressing:

    Article Title: Nuclear DNA Sensor IFI16 as Circulating Protein in Autoimmune Diseases Is a Signal of Damage that Impairs Endothelial Cells through High-Affinity Membrane Binding
    Article Snippet: Low passage human dermal fibroblasts, HDF (ATCC), mouse fibroblasts, 3T3 (ATCC), HeLa (ATCC) and HaCaT (ATCC) cells were grown in Dulbecco’s modified Eagle’s medium ( DMEM) (Sigma-Aldrich, Milan, Italy) supplemented with 10% fetal bovine serum and 2% Penicillin-Streptomycin solution Unless specified, all cells were grown at 37°C and 5% CO 2 . .. The entire coding sequence of the b isoform of human IFI16 was subcloned into the pET30a expression vector (Novagen, Madison, WI) containing an N-terminal histidine tag. ..

    Article Title: Effects of Hepatitis C core protein on mitochondrial electron transport and production of reactive oxygen species
    Article Snippet: .. Sequence of the cloned PCR fragment was confirmed by DNA sequencing and the fragment was then subcloned into a pET30a expression vector (Novagen, Gillstown, NJ). ..

    Article Title: Method for detecting cancer using CAPRIN-1 as a marker
    Article Snippet: .. Then the gene sequence of interest was inserted into a pET30a expression vector (Novagen) for Escherichia coli treated with Sac I and Xho I restriction enzymes. .. A His tag-fused recombinant protein can be produced using the vector.

    Article Title: A hexokinase from the oyster Crassostrea gigas is involved in immune recognition as a pattern recognition receptor.
    Article Snippet: Hexokinase (HK) is generally recognized as a crucial enzyme participating in glycolysis.. In the present study, a HK (named CgHK) was identified as a potential pattern recognition receptor (PRR) from the Pacific oyster Crassostrea gigas.. The open reading frame (ORF) of CgHK was of 1395 bp, encoding a peptide of 464 amino acids with one Hexokinase_1 domain and one Hexokinase_2 domain.

    Article Title: The Oligonucleotide Binding (OB)-Fold Domain of KREPA4 Is Essential for Stable Incorporation into Editosomes
    Article Snippet: .. pET30a expression vector (Novagen) was used to construct the N-terminal 6X His-tagged full length KREPA6. ..

    Article Title: Molecular and functional characterization of odorant-binding protein genes in Holotrichia oblita Faldermann.
    Article Snippet: Accepted Manuscript Molecular and functional characterization of odorant-binding protein genes in Holotrichia oblita Faldermann Chao-Qun Wang, Jin-Qiao Li, Er-Tao Li, Nyamwasa Innocent, Ke-Bin Li, Shuai Zhang, Yu Peng, Zhao-Jun Wei, Jiao Yin PII: S0141-8130(19)31538-7 DOI: https://doi.org/10.1016/j.ijbiomac.2019.06.013 Reference: BIOMAC 12519 To appear in: International Journal of Biological Macromolecules Received date: 28 February 2019 Revised date: 19 May 2019 Accepted date: 3 June 2019 Please cite this article as: C.-Q.. Wang, J.-Q.. Li, E.-T. Li, et al., Molecular and functional characterization of odorant-binding protein genes in Holotrichia oblita Faldermann, International Journal of Biological Macromolecules, https://doi.org/10.1016/ j.ijbiomac.2019.06.013 This is a PDF file of an unedited manuscript that has been accepted for publication.

    Article Title: A natural fusion of flavodiiron, rubredoxin, and rubredoxin oxidoreductase domains is a self-sufficient water-forming oxidase of Trichomonas vaginalis
    Article Snippet: .. All resulting PCR products were cloned into the pET30a expression vector (Novagen, EMD Millipore). .. All proteins in this study were overproduced in One Shot BL21 Star (DE3) chemically competent E. coli (Thermo Fisher [formerly Invitrogen]; catalog no.: C601003).

    Clone Assay:

    Article Title: Effects of Hepatitis C core protein on mitochondrial electron transport and production of reactive oxygen species
    Article Snippet: .. Sequence of the cloned PCR fragment was confirmed by DNA sequencing and the fragment was then subcloned into a pET30a expression vector (Novagen, Gillstown, NJ). ..

    Article Title: A natural fusion of flavodiiron, rubredoxin, and rubredoxin oxidoreductase domains is a self-sufficient water-forming oxidase of Trichomonas vaginalis
    Article Snippet: .. All resulting PCR products were cloned into the pET30a expression vector (Novagen, EMD Millipore). .. All proteins in this study were overproduced in One Shot BL21 Star (DE3) chemically competent E. coli (Thermo Fisher [formerly Invitrogen]; catalog no.: C601003).

    Polymerase Chain Reaction:

    Article Title: Effects of Hepatitis C core protein on mitochondrial electron transport and production of reactive oxygen species
    Article Snippet: .. Sequence of the cloned PCR fragment was confirmed by DNA sequencing and the fragment was then subcloned into a pET30a expression vector (Novagen, Gillstown, NJ). ..

    Article Title: A hexokinase from the oyster Crassostrea gigas is involved in immune recognition as a pattern recognition receptor.
    Article Snippet: Hexokinase (HK) is generally recognized as a crucial enzyme participating in glycolysis.. In the present study, a HK (named CgHK) was identified as a potential pattern recognition receptor (PRR) from the Pacific oyster Crassostrea gigas.. The open reading frame (ORF) of CgHK was of 1395 bp, encoding a peptide of 464 amino acids with one Hexokinase_1 domain and one Hexokinase_2 domain.

    Article Title: A natural fusion of flavodiiron, rubredoxin, and rubredoxin oxidoreductase domains is a self-sufficient water-forming oxidase of Trichomonas vaginalis
    Article Snippet: .. All resulting PCR products were cloned into the pET30a expression vector (Novagen, EMD Millipore). .. All proteins in this study were overproduced in One Shot BL21 Star (DE3) chemically competent E. coli (Thermo Fisher [formerly Invitrogen]; catalog no.: C601003).

    DNA Sequencing:

    Article Title: Effects of Hepatitis C core protein on mitochondrial electron transport and production of reactive oxygen species
    Article Snippet: .. Sequence of the cloned PCR fragment was confirmed by DNA sequencing and the fragment was then subcloned into a pET30a expression vector (Novagen, Gillstown, NJ). ..

    Plasmid Preparation:

    Article Title: Method for detecting cancer using CAPRIN-1 as a marker
    Article Snippet: .. Then the gene sequence of interest was inserted into a pET30a expression vector (Novagen) for Escherichia coli treated with Sac I and Xho I restriction enzymes. .. A His tag-fused recombinant protein can be produced using the vector.

    Article Title: A hexokinase from the oyster Crassostrea gigas is involved in immune recognition as a pattern recognition receptor.
    Article Snippet: Hexokinase (HK) is generally recognized as a crucial enzyme participating in glycolysis.. In the present study, a HK (named CgHK) was identified as a potential pattern recognition receptor (PRR) from the Pacific oyster Crassostrea gigas.. The open reading frame (ORF) of CgHK was of 1395 bp, encoding a peptide of 464 amino acids with one Hexokinase_1 domain and one Hexokinase_2 domain.

    Article Title: The Oligonucleotide Binding (OB)-Fold Domain of KREPA4 Is Essential for Stable Incorporation into Editosomes
    Article Snippet: .. pET30a expression vector (Novagen) was used to construct the N-terminal 6X His-tagged full length KREPA6. ..

    Article Title: A natural fusion of flavodiiron, rubredoxin, and rubredoxin oxidoreductase domains is a self-sufficient water-forming oxidase of Trichomonas vaginalis
    Article Snippet: .. All resulting PCR products were cloned into the pET30a expression vector (Novagen, EMD Millipore). .. All proteins in this study were overproduced in One Shot BL21 Star (DE3) chemically competent E. coli (Thermo Fisher [formerly Invitrogen]; catalog no.: C601003).

    Purification:

    Article Title: A hexokinase from the oyster Crassostrea gigas is involved in immune recognition as a pattern recognition receptor.
    Article Snippet: Hexokinase (HK) is generally recognized as a crucial enzyme participating in glycolysis.. In the present study, a HK (named CgHK) was identified as a potential pattern recognition receptor (PRR) from the Pacific oyster Crassostrea gigas.. The open reading frame (ORF) of CgHK was of 1395 bp, encoding a peptide of 464 amino acids with one Hexokinase_1 domain and one Hexokinase_2 domain.

    other:

    Article Title: Crystal structure and biochemical characterization of malate dehydrogenase from Metallosphaera sedula.
    Article Snippet: Metallosphaera sedula is a thermoacidophilic autotrophic archaeon and known to utilize the 3hydroxypropionate/4-hydroxybutyrate cycle (3-HP/4-HB cycle) as a carbon fixation pathway.. The 3HP/4-HB cycle in M. sedula is associated with central metabolism, and malate dehydrogenase (MDH) is an enzyme involved in the central metabolism that converts malate to oxaloacetate.. To elucidate the enzymatic properties of MDH from M. sedula (MsMDH), we determined the crystal structure of MsMDH as a complex with NADþ and a ternary complex with malate and NADþ.

    Construct:

    Article Title: The Oligonucleotide Binding (OB)-Fold Domain of KREPA4 Is Essential for Stable Incorporation into Editosomes
    Article Snippet: .. pET30a expression vector (Novagen) was used to construct the N-terminal 6X His-tagged full length KREPA6. ..

    Recombinant:

    Article Title: Molecular and functional characterization of odorant-binding protein genes in Holotrichia oblita Faldermann.
    Article Snippet: Accepted Manuscript Molecular and functional characterization of odorant-binding protein genes in Holotrichia oblita Faldermann Chao-Qun Wang, Jin-Qiao Li, Er-Tao Li, Nyamwasa Innocent, Ke-Bin Li, Shuai Zhang, Yu Peng, Zhao-Jun Wei, Jiao Yin PII: S0141-8130(19)31538-7 DOI: https://doi.org/10.1016/j.ijbiomac.2019.06.013 Reference: BIOMAC 12519 To appear in: International Journal of Biological Macromolecules Received date: 28 February 2019 Revised date: 19 May 2019 Accepted date: 3 June 2019 Please cite this article as: C.-Q.. Wang, J.-Q.. Li, E.-T. Li, et al., Molecular and functional characterization of odorant-binding protein genes in Holotrichia oblita Faldermann, International Journal of Biological Macromolecules, https://doi.org/10.1016/ j.ijbiomac.2019.06.013 This is a PDF file of an unedited manuscript that has been accepted for publication.

    Transformation Assay:

    Article Title: Molecular and functional characterization of odorant-binding protein genes in Holotrichia oblita Faldermann.
    Article Snippet: Accepted Manuscript Molecular and functional characterization of odorant-binding protein genes in Holotrichia oblita Faldermann Chao-Qun Wang, Jin-Qiao Li, Er-Tao Li, Nyamwasa Innocent, Ke-Bin Li, Shuai Zhang, Yu Peng, Zhao-Jun Wei, Jiao Yin PII: S0141-8130(19)31538-7 DOI: https://doi.org/10.1016/j.ijbiomac.2019.06.013 Reference: BIOMAC 12519 To appear in: International Journal of Biological Macromolecules Received date: 28 February 2019 Revised date: 19 May 2019 Accepted date: 3 June 2019 Please cite this article as: C.-Q.. Wang, J.-Q.. Li, E.-T. Li, et al., Molecular and functional characterization of odorant-binding protein genes in Holotrichia oblita Faldermann, International Journal of Biological Macromolecules, https://doi.org/10.1016/ j.ijbiomac.2019.06.013 This is a PDF file of an unedited manuscript that has been accepted for publication.



    Similar Products

    99
    New England Biolabs pet30a expression vector
    Pet30a Expression Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30a+expression+vector/NdeI/pmc11125983-59-46-53
    Average 99 stars, based on 1 article reviews
    pet30a expression vector - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    90
    GenScript corporation pet30a expression vectors
    Pet30a Expression Vectors, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30a+expression+vector/pet30a/pmc12124639-412-45-61
    Average 90 stars, based on 1 article reviews
    pet30a expression vectors - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Merck KGaA pet30a expression vector
    Pet30a Expression Vector, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30a+expression+vector/pet32a/pmc10772564-76-7-10
    Average 90 stars, based on 1 article reviews
    pet30a expression vector - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Promega pet30a prokaryotic expression vector
    Pet30a Prokaryotic Expression Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30a+expression+vector/pet30a+vector/bio_rxiv__2023__11__30__569188-225-6-10
    Average 90 stars, based on 1 article reviews
    pet30a prokaryotic expression vector - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies pet30a/txgh116δ1-18 expression vector
    Pet30a/Txgh116δ1 18 Expression Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30a+expression+vector/pm37180965-152-19-28
    Average 90 stars, based on 1 article reviews
    pet30a/txgh116δ1-18 expression vector - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Millipore pet30a expression vector
    Pet30a Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30a+expression+vector/pet28a/pmc03464273-227-0-3
    Average 90 stars, based on 1 article reviews
    pet30a expression vector - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results